WNT7A Polyclonal antibody
WNT7A Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 27177-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, mouse kidney tissue, rat kidney tissue |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
27177-1-AP targets WNT7A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | WNT7A fusion protein Ag26060 相同性解析による交差性が予測される生物種 |
Full Name | wingless-type MMTV integration site family, member 7A |
Calculated molecular weight | 39 kDa |
Observed molecular weight | 30-39 kDa |
GenBank accession number | BC008811 |
Gene symbol | WNT7A |
Gene ID (NCBI) | 7476 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WNT7A belongs to the Wnt family. WNT7A plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PMID: 16826533). WNT7A is required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PMID:30026314). WNT7A is highly expressed in basal cells and is secreted in abundance by basal cells (PMID: 36318668).
Protocols
Product Specific Protocols | |
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WB protocol for WNT7A antibody 27177-1-AP | Download protocol |
IF protocol for WNT7A antibody 27177-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |