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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WNT7A Polyclonal antibody

WNT7A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27177-1-AP

Synonyms

WNT7A/B, Protein Wnt-7a, Protein Wnt 7a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WNT7A Polyclonal antibody (27177-1-AP)

    WB analysis using 27177-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27177-1-AP (WNT7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using WNT7A Polyclonal antibody (27177-1-AP)

    IP experiment of mouse kidney using 27177-1-AP

    IP result of anti-WNT7A (IP:27177-1-AP, 4ug; Detection:27177-1-AP 1:2000) with mouse kidney tissue lysate 2160 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WNT7A Polyclonal antibody (27177-1-AP)

    IF Staining of HepG2 using 27177-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (27177-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse kidney tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

27177-1-AP targets WNT7A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT7A fusion protein Ag26060 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 7A
Calculated molecular weight 39 kDa
Observed molecular weight 30-39 kDa
GenBank accession numberBC008811
Gene symbol WNT7A
Gene ID (NCBI) 7476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WNT7A belongs to the Wnt family. WNT7A plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PMID: 16826533). WNT7A is required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PMID:30026314). WNT7A is highly expressed in basal cells and is secreted in abundance by basal cells (PMID: 36318668).

Protocols

Product Specific Protocols
WB protocol for WNT7A antibody 27177-1-APDownload protocol
IF protocol for WNT7A antibody 27177-1-APDownload protocol
IP protocol for WNT7A antibody 27177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

EMBO Rep

Ehbp1 orchestrates orderly sorting of Wnt/Wingless to the basolateral and apical cell membranes

Authors - Yuan Gao
View Article