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  • Featured Product
  • KD/KO Validated

WRAP53 Polyclonal antibody

WRAP53 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14761-1-AP

Synonyms

TCAB1, WDR79, WRAP53

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using WRAP53 Polyclonal antibody (14761-1-AP)

    WB analysis using 14761-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    WB analysis of human kidney using 14761-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using WRAP53 Polyclonal antibody (14761-1-AP)

    WB analysis of HeLa using 14761-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using WRAP53 Polyclonal antibody (14761-1-AP)

    WB analysis of Raji using 14761-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14761-1-AP (WRAP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using WRAP53 Polyclonal antibody (14761-1-AP)

    IP experiment of HeLa using 14761-1-AP

    IP result of anti-WRAP53 (IP:14761-1-AP, 4ug; Detection:14761-1-AP 1:300) with HeLa cells lysate 2480ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of mouse testis using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of mouse testis using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of mouse testis using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of mouse testis using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14761-1-AP (WRAP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human ovary using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human ovary using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human heart using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human heart using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human kidney using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human kidney using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human placenta using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human placenta using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human testis using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human testis using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human skin using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human skin using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human brain using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human brain using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human spleen using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using WRAP53 Polyclonal antibody (14761-1-AP)

    IHC staining of human spleen using 14761-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14761-1-AP (WRAP53 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inRaji cells, human kidney tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, human heart tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14761-1-AP targets WRAP53 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WRAP53 fusion protein Ag6522 相同性解析による交差性が予測される生物種
Full Name WD repeat containing, antisense to TP53
Calculated molecular weight 59 kDa
Observed molecular weight 72-75 kDa
GenBank accession numberBC002336
Gene symbol WRAP53
Gene ID (NCBI) 55135
RRIDAB_1939806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for WRAP53 antibody 14761-1-APDownload protocol
IHC protocol for WRAP53 antibody 14761-1-APDownload protocol
IP protocol for WRAP53 antibody 14761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Genes Dev

The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair.

Authors - Sofia Henriksson
View Article
humanWB

Nat Chem Biol

Observation of processive telomerase catalysis using high-resolution optical tweezers.

Authors - Eric M Patrick
View Article
humanWB

Oncogenesis

RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.

Authors - Qunsong Tan
  • KD Validated
View Article
WB

Aging (Albany NY)

Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism.

Authors - Xing-Hua Pan
View Article
humanWB,IF

RNA Biol

Phosphorylation of the Cajal body protein WRAP53β by ATM promotes its involvement in the DNA damage response.

Authors - Christos Coucoravas
  • KD Validated
View Article
humanWB

Cancer Cell Int

Suppression of TCAB1 expression induced cellular senescence by lessening proteasomal degradation of p21 in cancer cells.

Authors - Jing Niu
View Article