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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WSB1 Polyclonal antibody (11666-1-AP)

    WB analysis using 11666-1-AP (same clone as 11666-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11666-1-AP (WSB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WSB1 Polyclonal antibody (11666-1-AP)

    IHC staining of human prostate cancer using 11666-1-AP (same clone as 11666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11666-1-AP (WSB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WSB1 Polyclonal antibody (11666-1-AP)

    IF Staining of HepG2 using 11666-1-AP (same clone as 11666-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WSB1 antibody (11666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11666-1-PBS in a different storage buffer formulation.

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WSB1 Polyclonal antibody, PBS Only

WSB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11666-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11666-1-PBS targets WSB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WSB1 fusion protein Ag2211 相同性解析による交差性が予測される生物種
Full Name WD repeat and SOCS box-containing 1
Calculated molecular weight 421 aa, 47 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC021110
Gene Symbol WSB1
Gene ID (NCBI) 26118
RRIDAB_10949076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6I7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

WSB1 (or SWIP1) gene encodes WD repeat and SOCS box-containing protein 1, a member of the WD-protein subfamily. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding 3 distinct isoforms have been found for this gene. WSB1 serves as a probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WSB1 has been reported (PMID: 15965468), initiated by the interaction of the two components (PMID: 18093972). WSB1 is also a part of an E3 ubiquitin ligase for the thyroid-hormone-activating type 2 iodothyronine deiodinase (D2) (PMID: 15965468).