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Species Reactivity

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  • Featured Product
  • KD/KO Validated

WT1 Polyclonal antibody

WT1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 12609-1-AP

Synonyms

Wilms tumor protein, Wilms tumor 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of K-562 using 12609-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of MCF-7 using 12609-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of human liver using 12609-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of rat kidney using 12609-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse kidney tissue using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of mouse kidney using 12609-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of A431 cells using WT1 Polyclonal antibody (12609-1-AP)

    WB analysis of A431 using 12609-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12609-1-AP (WT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using WT1 Polyclonal antibody (12609-1-AP)

    IP experiment of K-562 using 12609-1-AP

    IP result of anti-WT1 (IP:12609-1-AP, 4ug; Detection:12609-1-AP 1:1000) with K-562 cells lysate 1360 ug.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using WT1 Polyclonal antibody (12609-1-AP)

    IF Staining of K-562 using 12609-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using WT1 antibody (12609-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K-562 cells using WT1 Polyclonal antibody (12609-1-AP)

    FC experiment of K-562 using 12609-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human WT1 (12609-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inK-562 cells, A431 cells, human liver tissue, MCF-7 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inK-562 cells
Positive IF/ICC detected inK-562 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12609-1-AP targets WT1 in WB, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, bovine, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WT1 fusion protein Ag3337 相同性解析による交差性が予測される生物種
Full Name Wilms tumor 1
Calculated molecular weight 449 aa, 49 kDa, 57 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC032861
Gene symbol WT1
Gene ID (NCBI) 7490
RRIDAB_2216225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.

Protocols

Product Specific Protocols
WB protocol for WT1 antibody 12609-1-APDownload protocol
IF protocol for WT1 antibody 12609-1-APDownload protocol
IP protocol for WT1 antibody 12609-1-APDownload protocol
FC protocol for WT1 antibody 12609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.

Authors - Michael Heider
View Article
mouseWB,IF

Mol Psychiatry

Brain-specific Wt1 deletion leads to depressive-like behaviors in mice via the recruitment of Tet2 to modulate Epo expression.

Authors - Fen Ji
  • KO Validated
View Article
mousechIP

EMBO Mol Med

Inhibition of Aurora Kinase B attenuates fibroblast activation and pulmonary fibrosis.

Authors - Rajesh K Kasam
View Article
humanWB

Oncogene

YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells.

Authors - G Hua
View Article
mouseWB

Oncogene

USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.

Authors - Juntae Kwon
View Article
humanIF

Elife

Induction of Sertoli-like cells from human fibroblasts by NR5A1 and GATA4.

Authors - Jianlin Liang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sandrine (Verified Customer) (12-17-2021)

clean signal for IP and western blot. Good relative enrichment for ChIP assays.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation, ChIP
  • Cell Tissue Type: kidney tissue/Podocytes
FH

K (Verified Customer) (12-20-2020)

This Ab worked well for IHC (1:200) but I could't get satisfactory results for western blotting.

  • Applications: Western Blot, Immunohistochemistry
  • Cell Tissue Type: mouse liver tissues. rat liver and human liver cells