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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WT1 Recombinant antibody (82525-1-RR)

    WB analysis using 82525-1-RR (same clone as 82525-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82525-1-RR (WT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using WT1 Recombinant antibody (82525-1-RR)

    IP experiment of K-562 using 82525-1-RR (same clone as 82525-1-PBS)

    IP result of anti-WT1 (IP:82525-1-RR, 4ug; Detection:82525-1-RR 1:1000) with K-562 cells lysate 1600 ug. This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WT1 Recombinant antibody (82525-1-RR)

    IHC staining of mouse testis using 82525-1-RR (same clone as 82525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82525-1-RR (WT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WT1 Recombinant antibody (82525-1-RR)

    IHC staining of mouse testis using 82525-1-RR (same clone as 82525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82525-1-RR (WT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82525-1-RR

    Affinity and Kinetic Characterization of 82525-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82525-1-RR against Human WT1 were performed. The affinity constant is 0.912 nM.

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WT1 Recombinant antibody, PBS Only

WT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82525-1-PBS
Clone No.2J13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Wilms tumor protein, Wilms tumor 1, 2J13

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82525-1-PBS targets WT1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WT1 fusion protein Ag19541 相同性解析による交差性が予測される生物種
Full Name Wilms tumor 1
Calculated molecular weight 449 aa, 49 kDa, 57 kDa
Observed molecular weight 52-55 kDa
GenBank accession numberBC032861
Gene Symbol WT1
Gene ID (NCBI) 7490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19544
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for the normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. The function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has a lower affinity for DNA and can bind RNA. WT1 exists some isoforms with a range of molecular weight is 33-50 kDa.