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  • Featured Product
  • KD/KO Validated

WTAP Polyclonal antibody, PBS Only

WTAP Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10200-1-PBS

Synonyms

Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, Mum2, KIAA0105, FL2D

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using WTAP Polyclonal antibody (10200-1-AP)

    WB analysis of HeLa using 10200-1-AP (same clone as 10200-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WTAP Polyclonal antibody (10200-1-AP)

    WB analysis using 10200-1-AP (same clone as 10200-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using WTAP Polyclonal antibody (10200-1-AP)

    WB analysis of Jurkat cells using 10200-1-AP (same clone as 10200-1-PBS)

    WB result of WTAP specific antibody (10200-1-AP, 1:6000) with si-control and si-WTAP transfected Jurkat cells. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WTAP Polyclonal antibody (10200-1-AP)

    WB analysis using 10200-1-AP (same clone as 10200-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10200-1-AP (WTAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using WTAP Polyclonal antibody (10200-1-AP)

    IP experiment of HEK-293 using 10200-1-AP (same clone as 10200-1-PBS)

    IP result of anti-WTAP (IP:10200-1-AP, 3ug; Detection:10200-1-AP 1:500) with HEK-293 cells lysate 2500ug. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using WTAP Polyclonal antibody (10200-1-AP)

    IP experiment of Jurkat using 10200-1-AP (same clone as 10200-1-PBS)

    IP result of anti-WTAP (IP:10200-1-AP, 4ug; Detection:10200-1-AP 1:8000) with Jurkat cells lysate 2280 ug. This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using WTAP Polyclonal antibody (10200-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 10200-1-AP (same clone as 10200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10200-1-AP (WTAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using WTAP Polyclonal antibody (10200-1-AP)

    IHC staining of human stomach using 10200-1-AP (same clone as 10200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10200-1-AP (WTAP antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10200-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10200-1-PBS targets WTAP in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WTAP fusion protein Ag0259 相同性解析による交差性が予測される生物種
Full Name Wilms tumor 1 associated protein
Calculated molecular weight 45 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000383
Gene symbol WTAP
Gene ID (NCBI) 9589
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 50-55 kDa due to phosphorylation.