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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WTAP Monoclonal antibody (60188-1-Ig)

    WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WTAP Monoclonal antibody (60188-1-Ig)

    WB analysis using 60188-1-Ig (same clone as 60188-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60188-1-Ig (WTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using WTAP Monoclonal antibody (60188-1-Ig)

    IP experiment of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

    IP result of anti-WTAP (IP:60188-1-Ig, 4ug; Detection:10200-1-AP 1:8000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using WTAP Monoclonal antibody (60188-1-Ig)

    IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using WTAP Monoclonal antibody (60188-1-Ig)

    IHC staining of human colon using 60188-1-Ig (same clone as 60188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60188-1-Ig (WTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WTAP Monoclonal antibody (60188-1-Ig)

    IF Staining of HeLa using 60188-1-Ig (same clone as 60188-1-PBS)

    Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 60188-1-Ig(WTAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60188-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

WTAP Monoclonal antibody, PBS Only

WTAP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60188-1-PBS
Clone No.4A10G9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Female lethal(2)D homolog, hFL(2)D, KIAA0105, WT1 associated protein, WTAP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60188-1-PBS targets WTAP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen WTAP fusion protein Ag0259 相同性解析による交差性が予測される生物種
Full Name Wilms tumor 1 associated protein
Calculated molecular weight 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC000383
Gene Symbol WTAP
Gene ID (NCBI) 9589
RRIDAB_10859484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15007
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.