- Featured Product
- KD/KO Validated
WWOX Polyclonal antibody
WWOX Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 15299-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, MCF-7 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, mouse testis tissue |
Positive IP detected in | mouse skeletal muscle tissue |
Positive IHC detected in | human liver cancer tissue, mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 5 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
15299-1-AP targets WWOX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | WWOX fusion protein Ag7555 相同性解析による交差性が予測される生物種 |
Full Name | WW domain containing oxidoreductase |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 46 kDa |
GenBank accession number | BC003184 |
Gene symbol | WWOX |
Gene ID (NCBI) | 51741 |
RRID | AB_2216389 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for WWOX antibody 15299-1-AP | Download protocol |
IHC protocol for WWOX antibody 15299-1-AP | Download protocol |
IP protocol for WWOX antibody 15299-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hum Mutat Early infantile onset epileptic encephalopathy 28 due to a homozygous microdeletion involving the WWOX gene in a region of uniparental disomy. | ||
Front Oncol WWOX-rs13338697 genotype predicts therapeutic efficacy of ADI-PEG 20 for patients with advanced hepatocellular carcinoma
| ||
Molecules Evodiamine Exerts an Anti-Hepatocellular Carcinoma Activity through a WWOX-Dependent Pathway. | ||
Int J Mol Med WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines.
| ||
Oncol Lett Expression and clinical significance of WWOX, Elf5, Snail1 and EMT related factors in epithelial ovarian cancer. | ||
Int J Cancer Remote modulation of WWOX by an intronic variant associated with survival of Chinese gastric cancer patients
|