Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, mouse testis tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue, mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
15299-1-AP targets WWOX in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | WWOX fusion protein Ag7555 相同性解析による交差性が予測される生物種 |
| Full Name | WW domain containing oxidoreductase |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC003184 |
| Gene Symbol | WWOX |
| Gene ID (NCBI) | 51741 |
| RRID | AB_2216389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZC7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WWOX antibody 15299-1-AP | Download protocol |
| IHC protocol for WWOX antibody 15299-1-AP | Download protocol |
| IP protocol for WWOX antibody 15299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hum Mutat Early infantile onset epileptic encephalopathy 28 due to a homozygous microdeletion involving the WWOX gene in a region of uniparental disomy. | ||
Front Oncol WWOX-rs13338697 genotype predicts therapeutic efficacy of ADI-PEG 20 for patients with advanced hepatocellular carcinoma
| ||
Molecules Evodiamine Exerts an Anti-Hepatocellular Carcinoma Activity through a WWOX-Dependent Pathway. | ||
Int J Mol Med WWOX induces apoptosis and inhibits proliferation in cervical cancer and cell lines.
| ||
Oncol Lett Expression and clinical significance of WWOX, Elf5, Snail1 and EMT related factors in epithelial ovarian cancer. | ||
Int J Cancer Remote modulation of WWOX by an intronic variant associated with survival of Chinese gastric cancer patients
|