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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WWOX Monoclonal antibody, PBS Only

WWOX Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8F4

Cat no : 67725-1-PBS

Synonyms

D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WWOX

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using WWOX Monoclonal antibody (67725-1-Ig)

    WB analysis using 67725-1-Ig (same clone as 67725-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67725-1-Ig (WWOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using WWOX Monoclonal antibody (67725-1-Ig)

    IHC staining of mouse testis using 67725-1-Ig (same clone as 67725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67725-1-Ig (WWOX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67725-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67725-1-PBS targets WWOX in WB, IHC, ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Reactivity Human, rat, pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen WWOX fusion protein Ag7969 相同性解析による交差性が予測される生物種
Full Name WW domain containing oxidoreductase
Calculated molecular weight 47 kDa
Observed molecular weight40 kDa
GenBank accession numberBC003184
Gene symbol WWOX
Gene ID (NCBI) 51741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.