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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using WWP1 Polyclonal antibody (28689-1-AP)

    WB analysis of HepG2 using 28689-1-AP (same clone as 28689-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using WWP1 Polyclonal antibody (28689-1-AP)

    WB analysis of HeLa using 28689-1-AP (same clone as 28689-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using WWP1 Polyclonal antibody (28689-1-AP)

    WB analysis of MCF-7 using 28689-1-AP (same clone as 28689-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using WWP1 Polyclonal antibody (28689-1-AP)

    WB analysis of A431 using 28689-1-AP (same clone as 28689-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 28689-1-AP (WWP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WWP1 Polyclonal antibody (28689-1-AP)

    IHC staining of human breast cancer using 28689-1-AP (same clone as 28689-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using WWP1 Polyclonal antibody (28689-1-AP)

    IHC staining of human breast cancer using 28689-1-AP (same clone as 28689-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WWP1 Polyclonal antibody (28689-1-AP)

    IHC staining of human liver cancer using 28689-1-AP (same clone as 28689-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using WWP1 Polyclonal antibody (28689-1-AP)

    IHC staining of human liver cancer using 28689-1-AP (same clone as 28689-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28689-1-AP (WWP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28689-1-PBS in a different storage buffer formulation.

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WWP1 Polyclonal antibody, PBS Only

WWP1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28689-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28689-1-PBS targets WWP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WWP1 fusion protein Ag30161 相同性解析による交差性が予測される生物種
Full Name WW domain containing E3 ubiquitin protein ligase 1
Calculated molecular weight 922 aa, 105 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC036065
Gene Symbol WWP1
Gene ID (NCBI) 11059
RRIDAB_2881195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0M0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.