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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WWP1 Monoclonal antibody (67804-1-Ig)

    WB analysis using 67804-1-Ig (same clone as 67804-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67804-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HaCaT cells using WWP1 Monoclonal antibody (67804-1-Ig)

    WB analysis of HaCaT using 67804-1-Ig (same clone as 67804-1-PBS)

    HaCaT cells were subjected to SDS PAGE followed by western blot with 67804-1-Ig (WWP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67804-1-PBS in a different storage buffer formulation.

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WWP1 Monoclonal antibody, PBS Only

WWP1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67804-1-PBS
Clone No.2G8G11

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

AIP5, DKFZp434D2111, hSDRP1, Tiul1, WW domain containing protein 1, WWP1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67804-1-PBS targets WWP1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen WWP1 fusion protein Ag30171 相同性解析による交差性が予測される生物種
Full Name WW domain containing E3 ubiquitin protein ligase 1
Calculated molecular weight 922 aa, 105 kDa
Observed molecular weight 95-105 kDa
GenBank accession numberBC036065
Gene Symbol WWP1
Gene ID (NCBI) 11059
RRIDAB_2918567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0M0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.