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KD/KO Validated

XBP-1U specific Polyclonal antibody

Name: XBP-1U specific Polyclonal antibody
Brand: Proteintech
SKU: 25997-1-AP

XBP-1U specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 25997-1-AP

Synonyms

XBP1, X-box-binding protein 1, luminal form, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1

Validation Data Gallery

Filter:

AllWBIHCFC (Intra)

WB analysis of rat spleen using 25997-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 25997-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 25997-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25997-1-AP (XBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 25997-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25997-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 25997-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 25997-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 25997-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 25997-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 25997-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 25997-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25997-1-AP (XBP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HepG2 using 25997-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XBP-1U specific (25997-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 25997-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	rat spleen tissue, mouse spleen tissue
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 43 publications below
IHC	See 6 publications below
IF	See 2 publications below

Product Information

25997-1-AP targets XBP-1U specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	XBP-1U specific fusion protein Ag21714 相同性解析による交差性が予測される生物種
Full Name	X-box binding protein 1
Calculated molecular weight	261 aa, 29 kDa
Observed molecular weight	32 kDa
GenBank accession number	BC000938
Gene symbol	XBP1
Gene ID (NCBI)	7494
RRID	AB_2880326
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

X-box-binding protein 1 (XBP1), also named as TREB5, is a 261 amino acid protein, which contains one bZIP domain and belongs to the bZIP family. XBP1 localizes in the nucleus and as a transcription factor is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The molecular weight of protein generated from the spliced XBP1 mRNA is 54 kDa and protein generated from unspliced Xbp1 mRNA is 33 kDa. This antibody 25997-1-AP specially recognizes isoform XBP-1U.

Protocols

Product Specific Protocols
WB protocol for XBP-1U specific antibody 25997-1-AP	Download protocol
IHC protocol for XBP-1U specific antibody 25997-1-AP	Download protocol
FC protocol for XBP-1U specific antibody 25997-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Extracell Vesicles A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells. Authors - Charles P Hinzman View Article
mouse	WB	Hepatology Farnesoid X receptor signaling activates the hepatic X-box binding protein 1 pathway in vitro and in mice. Authors - Xiaoying Liu View Article
human	IHC,IF	Sci Adv Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library. Authors - Can Huang View Article
mouse	WB	Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. Authors - Weimin Yu View Article
pig	WB	Autophagy Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy. Authors - Sheng-Li Ming View Article
human	WB	Oncogene Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells. Authors - Yifei Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XBP-1U specific Polyclonal antibody

XBP-1U specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of rat spleen using 25997-1-AP

WB analysis of rat spleen using 25997-1-AP

WB analysis of mouse spleen using 25997-1-AP

IHC staining of human breast cancer using 25997-1-AP

IHC staining of human colon cancer using 25997-1-AP

IHC staining of human colon cancer using 25997-1-AP

IHC staining of human liver cancer using 25997-1-AP

IHC staining of human liver cancer using 25997-1-AP

IHC staining of mouse liver using 25997-1-AP

IHC staining of mouse liver using 25997-1-AP

IHC staining of human breast cancer using 25997-1-AP

FC experiment of HepG2 using 25997-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.

Farnesoid X receptor signaling activates the hepatic X-box binding protein 1 pathway in vitro and in mice.

Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.

Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.