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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XBP1S-specific Recombinant antibody (83959-5-RR)

    WB analysis using 83959-5-RR (same clone as 83959-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83959-5-RR (XBP1S-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using XBP1S-specific Recombinant antibody (83959-5-RR)

    IHC staining of mouse liver using 83959-5-RR (same clone as 83959-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-5-RR (XBP1S-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Ramos cells using XBP1S-specific Recombinant antibody (83959-5-RR)

    FC experiment of Ramos using 83959-5-RR (same clone as 83959-5-PBS)

    1x10^6 Ramos cells were intracellularly stained with 0.25 ug XBP1S-specific Recombinant antibody (83959-5-RR, Clone:241004B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83959-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83959-5-RR

    Affinity and Kinetic Characterization of 83959-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83959-5-RR against Human XBP1S-specific were performed. The affinity constant is 1.08 nM.

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XBP1S-specific Recombinant antibody, PBS Only

XBP1S-specific Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83959-5-PBS
Clone No.241004B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

XBP1, 241004B6, Tax-responsive element-binding protein 5, TREB-5, X box binding protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83959-5-PBS targets XBP1S-specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen XBP1S-specific fusion protein Ag21703 相同性解析による交差性が予測される生物種
Full Name X-box binding protein 1
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight60 kDa
GenBank accession numberBC000938
Gene Symbol XBP1
Gene ID (NCBI) 7494
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17861
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The calculated molecular weight of protein generated from the unspliced XBP1 isoform XBP1U mRNA is 33 kDa, and protein generated from spliced XBP1 isoform XBP1S mRNA is 54 kDa. This antibody specially recognizes the XBP1S isoform.