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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XCL1 Polyclonal antibody

XCL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26605-1-AP

Synonyms

Lymphotactin, Small-inducible cytokine C1, SCM-1-alpha, Cytokine SCM-1, C motif chemokine 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XCL1 Polyclonal antibody (26605-1-AP)

    WB analysis using 26605-1-AP

    Untreated, PMA, Ionomycin and Brefeldin A treated NK-92 cells were subjected to SDS PAGE followed by western blot with 26605-1-AP (Lymphotactin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using XCL1 Polyclonal antibody (26605-1-AP)

    IHC staining of human tonsillitis using 26605-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26605-1-AP (XCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inPMA, Ionomycin and Brefeldin A treated NK-92 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26605-1-AP targets XCL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XCL1 fusion protein Ag24258 相同性解析による交差性が予測される生物種
Full Name chemokine (C motif) ligand 1
Calculated molecular weight 114 aa, 13 kDa
Observed molecular weight13 kDa
GenBank accession numberBC070309
Gene symbol XCL1
Gene ID (NCBI) 6375
RRIDAB_3669553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XCL1 also known as Lymphotactin, is a member of the chemokine superfamily. Chemokines function in inflammatory and immunological responses, inducing leukocyte migration and activation.

Protocols

Product Specific Protocols
WB protocol for XCL1 antibody 26605-1-APDownload protocol
IHC protocol for XCL1 antibody 26605-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols