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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XIRP2 Polyclonal antibody

XIRP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 11896-1-AP

Synonyms

Xin actin-binding repeat-containing protein 2, Xeplin, CMYA3, Cardiomyopathy-associated protein 3, Beta-xin

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using XIRP2 Polyclonal antibody (11896-1-AP)

    WB analysis of COLO 320 using 11896-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using XIRP2 Polyclonal antibody (11896-1-AP)

    WB analysis of Caco-2 using 11896-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using XIRP2 Polyclonal antibody (11896-1-AP)

    WB analysis of human heart using 11896-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using XIRP2 Polyclonal antibody (11896-1-AP)

    IHC staining of human skeletal muscle using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using XIRP2 Polyclonal antibody (11896-1-AP)

    IHC staining of human skeletal muscle using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using XIRP2 Polyclonal antibody (11896-1-AP)

    IHC staining of human heart using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using XIRP2 Polyclonal antibody (11896-1-AP)

    IHC staining of human heart using 11896-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using XIRP2 Polyclonal antibody (11896-1-AP)

    IF Staining of mouse heart using 11896-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using XIRP2 antibody (11896-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inCOLO 320 cells, Caco-2 cells, human heart tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11896-1-AP targets XIRP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XIRP2 fusion protein Ag2515 相同性解析による交差性が予測される生物種
Full Name xin actin-binding repeat containing 2
Calculated molecular weight 379 kDa
Observed molecular weight 260-300 kDa
GenBank accession numberBC022888
Gene symbol XIRP2
Gene ID (NCBI) 129446
RRIDAB_10951481
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for XIRP2 antibody 11896-1-APDownload protocol
IHC protocol for XIRP2 antibody 11896-1-APDownload protocol
IF protocol for XIRP2 antibody 11896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Elife

Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain

Authors - Elizabeth L Wagner
View Article
mouseWB,IF

Front Physiol

Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling.

Authors - Emily Farrell
View Article
humanWB,IF

Biology (Basel)

Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma

Authors - Dahuan Li
  • KD Validated
View Article