Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis of COLO 320 using 11896-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 11896-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11896-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 11896-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 11896-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 11896-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 11896-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse heart using 11896-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using XIRP2 antibody (11896-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

XIRP2 Polyclonal antibody

XIRP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 11896-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

Xin actin-binding repeat-containing protein 2, Xeplin, CMYA3, Cardiomyopathy-associated protein 3, Beta-xin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, Caco-2 cells, human heart tissue
Positive IHC detected in	human skeletal muscle tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

11896-1-AP targets XIRP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	XIRP2 fusion protein Ag2515 相同性解析による交差性が予測される生物種
Full Name	xin actin-binding repeat containing 2
Calculated molecular weight	379 kDa
Observed molecular weight	260-300 kDa
GenBank accession number	BC022888
Gene Symbol	XIRP2
Gene ID (NCBI)	129446
RRID	AB_10951481
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	A4UGR9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for XIRP2 antibody 11896-1-AP	Download protocol
IHC protocol for XIRP2 antibody 11896-1-AP	Download protocol
IF protocol for XIRP2 antibody 11896-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	Elife Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain Authors - Elizabeth L Wagner View Article
mouse	WB,IF	Front Physiol Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling. Authors - Emily Farrell View Article
human	WB,IF	Biology (Basel) Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma Authors - Dahuan Li KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of COLO 320 using 11896-1-AP

WB analysis of Caco-2 using 11896-1-AP

WB analysis of human heart using 11896-1-AP

IHC staining of human skeletal muscle using 11896-1-AP

IHC staining of human skeletal muscle using 11896-1-AP

IHC staining of human heart using 11896-1-AP