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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XK Polyclonal antibody

XK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12830-1-AP

Synonyms

Kell complex 37 kDa component, KX, Kx antigen, Membrane transport protein XK, X1k, XK, XK related protein 1, XKR1, XRG1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using XK Polyclonal antibody (12830-1-AP)

    WB analysis of mouse pancreas using 12830-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12830-1-AP (XK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using XK Polyclonal antibody (12830-1-AP)

    WB analysis of MCF-7 using 12830-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12830-1-AP (XK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using XK Polyclonal antibody (12830-1-AP)

    IHC staining of human pancreas cancer using 12830-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using XK Polyclonal antibody (12830-1-AP)

    IHC staining of human pancreas cancer using 12830-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12830-1-AP (XK Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse pancreas tissue, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12830-1-AP targets XK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XK fusion protein Ag3550 相同性解析による交差性が予測される生物種
Full Name X-linked Kx blood group (McLeod syndrome)
Calculated molecular weight 444 aa, 51 kDa
Observed molecular weight 45-51 kDa
GenBank accession numberBC036019
Gene symbol XK
Gene ID (NCBI) 7504
RRIDAB_2877887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XK, also named as XKR1 and XRG1, is responsible for the Kx blood group system. XK forms a functional complex with the Kell glycoprotein. It represents a crucial factor for the maintenance of normal muscle structure and function.

Protocols

Product Specific Protocols
WB protocol for XK antibody 12830-1-APDownload protocol
IHC protocol for XK antibody 12830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols