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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XPA Polyclonal antibody

XPA Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 16462-1-AP

Synonyms

XPAC, XP1, Xeroderma pigmentosum group A-complementing protein, DNA repair protein complementing XP-A cells

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of L02 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of L02 using 16462-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of MCF-7 using 16462-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of HEK-293 using 16462-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of HeLa using 16462-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of HeLa using 16462-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of HeLa using 16462-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of MCF-7 using 16462-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of L02 using 16462-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using XPA Polyclonal antibody (16462-1-AP)

    IP experiment of L02 using 16462-1-AP

    IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using XPA Polyclonal antibody (16462-1-AP)

    IF Staining of HepG2 using 16462-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XPA antibody (16462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using XPA Polyclonal antibody (16462-1-AP)

    FC experiment of HepG2 using 16462-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inL02 cells, HeLa cells, MCF-7 cells
Positive IP detected inL02 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16462-1-AP targets XPA in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XPA fusion protein Ag9536 相同性解析による交差性が予測される生物種
Full Name xeroderma pigmentosum, complementation group A
Calculated molecular weight374aa,42 kDa; 273aa,31 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC014965
Gene symbol XPA
Gene ID (NCBI) 7507
RRIDAB_2878261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).

Protocols

Product Specific Protocols
WB protocol for XPA antibody 16462-1-APDownload protocol
IF protocol for XPA antibody 16462-1-APDownload protocol
IP protocol for XPA antibody 16462-1-APDownload protocol
FC protocol for XPA antibody 16462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

PMID: 31249811

Authors - Ma-Sha Huang
View Article
humanWB

J Cancer

PMID: 31772670

Authors - Li-Ming Tan
View Article
mouseWB

Genes Dev

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Authors - Zhe Yang
View Article
human,mouseWB,IF

Adv Healthc Mater

Macromolecular NO-Donor Micelles for Targeted and Augmented Chemotherapy against Prostate Cancer

Authors - Jiaxin Chen
View Article
humanWB

Front Pharmacol

Triptolide enhances carboplatin-induced apoptosis by inhibiting nucleotide excision repair (NER) activity in melanoma

Authors - Geng Wang
View Article
zebrafishIF

Sci Total Environ

AHR-mediated DNA damage contributes to BaP-induced cardiac malformations in zebrafish

Authors - Hongmei Zou
View Article