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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XPNPEP3 Polyclonal antibody (15655-1-AP)

    WB analysis using 15655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using XPNPEP3 Polyclonal antibody (15655-1-AP)

    WB analysis using 15655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using XPNPEP3 Polyclonal antibody (15655-1-AP)

    WB analysis of human testis using 15655-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using XPNPEP3 Polyclonal antibody (15655-1-AP)

    WB analysis of human spleen using 15655-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using XPNPEP3 Polyclonal antibody (15655-1-AP)

    IP experiment of PC-3 using 15655-1-AP

    IP result of anti-XPNPEP3 (IP:15655-1-AP, 4ug; Detection:15655-1-AP 1:400) with PC-3 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using XPNPEP3 Polyclonal antibody (15655-1-AP)

    IHC staining of mouse liver using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using XPNPEP3 Polyclonal antibody (15655-1-AP)

    IHC staining of mouse liver using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using XPNPEP3 Polyclonal antibody (15655-1-AP)

    IHC staining of human oesophagus using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 15655-1-AP (XPNPEP3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using XPNPEP3 Polyclonal antibody (15655-1-AP)

    IHC staining of human oesophagus using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 15655-1-AP (XPNPEP3 Antibody) at dilution of 1:50 (under 40x lens).

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XPNPEP3 Polyclonal antibody

XPNPEP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15655-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

X Pro aminopeptidase 3, EC:3.4.11.9, APP3m, APP3c, APP3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, human testis tissue, human spleen tissue, PC-3 cells, 4T1 cells, mouse liver
Positive IP detected inPC-3 cells
Positive IHC detected inmouse liver tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15655-1-AP targets XPNPEP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XPNPEP3 fusion protein Ag8007 相同性解析による交差性が予測される生物種
Full Name X-prolyl aminopeptidase (aminopeptidase P) 3, putative
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC004989
Gene Symbol XPNPEP3
Gene ID (NCBI) 63929
RRIDAB_2288500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQH7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XPNPEP3 gene encodes X-prolyl aminopeptidase 3 which functions to remove the penultimate N-terminal Proline residue from nascent proteins and appears to play a role in ciliary function. CRC tissue microarray revealed increased XPNPEP3 expression in tumor compared to matched normal samples (PMID:29383790). Other study found that XPNPEP3 localizes to mitochondria in renal cells in vitro and to kidney tubules in a cell type-specific pattern, and mutations in the gene are associated with NPHP-like disorder (PMID:20179356).

Protocols

Product Specific Protocols
WB protocol for XPNPEP3 antibody 15655-1-APDownload protocol
IHC protocol for XPNPEP3 antibody 15655-1-APDownload protocol
IP protocol for XPNPEP3 antibody 15655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols