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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XPR1 Recombinant antibody (84902-1-RR)

    WB analysis using 84902-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84902-1-RR (XPR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84902-1-RR

    Affinity and Kinetic Characterization of 84902-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84902-1-RR against Human XPR1 were performed. The affinity constant is below 1 pM.

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XPR1 Recombinant antibody

XPR1 Recombinant Antibody for WB, ELISA
Cat No. 84902-1-RR
Clone No.241788A7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SYG1, Solute carrier family 53 member 1, SLC53A1, Protein SYG1 homolog, Phosphate exporter SLC53A1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHL-60 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84902-1-RR targets XPR1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen XPR1 fusion protein Ag5373 相同性解析による交差性が予測される生物種
Full Name xenotropic and polytropic retrovirus receptor
Calculated molecular weight 696 aa, 82 kDa
Observed molecular weight74 kDa
GenBank accession numberBC041142
Gene Symbol XPR1
Gene ID (NCBI) 9213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBH6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XPR1 is a receptor for xenotropic and polytropic murine leukemia retroviruses and a homolog of yeast Syg1 and plant Pi transporter PHO1 (PMID: 20633538). XPR1 has been identified as an atypical G-protein-coupled receptor. Xenotropic or polytropic retrovirus binding to XPR1 can disrupt the cAMP-mediated signaling function of Xpr1, leading to the apoptosis of infected cells (PMID: 22090134). A band of about 82-100 kDa is probably due to abnormal migration of the protein or post-translation modifications. In addition, a band of about 55-72 kDa may be isoforms and fragments.

Protocols

Product Specific Protocols
WB protocol for XPR1 antibody 84902-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols