• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XRCC2 Polyclonal antibody

XRCC2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20285-1-AP

Synonyms

DNA repair protein XRCC2, XRCC2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using XRCC2 Polyclonal antibody (20285-1-AP)

    WB analysis of K-562 using 20285-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20285-1-AP (XRCC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using XRCC2 Polyclonal antibody (20285-1-AP)

    WB analysis of HEK-293 using 20285-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20285-1-AP (XRCC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using XRCC2 Polyclonal antibody (20285-1-AP)

    IP experiment of K-562 using 20285-1-AP

    IP result of anti-XRCC2 (IP:20285-1-AP, 4ug; Detection:20285-1-AP 1:500) with K-562 cells lysate 3200ug.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inK-562 cells, A375 cells, HEK-293 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20285-1-AP is XRCC2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XRCC2 fusion protein Ag14011 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 2
Calculated molecular weight 280 aa, 32 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC042137
Gene symbol XRCC2
Gene ID (NCBI) 7516
RRIDAB_2878662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for XRCC2 antibody 20285-1-APDownload protocol
IP protocol for XRCC2 antibody 20285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development

Authors - Nazareno Bona
View Article