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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    WB analysis of HEK-293 using 66652-1-Ig (same clone as 66652-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    WB analysis of Raji using 66652-1-Ig (same clone as 66652-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    WB analysis of NIH/3T3 using 66652-1-Ig (same clone as 66652-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66652-1-Ig (XRCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    IF Staining of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66652-1-Ig (XRCC2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    IF Staining of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC2 antibody (66652-1-Ig, Clone: 3D9A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using XRCC2 Monoclonal antibody (66652-1-Ig)

    FC experiment of HepG2 using 66652-1-Ig (same clone as 66652-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRCC2 (66652-1-Ig, Clone:3D9A1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66652-1-PBS in a different storage buffer formulation.

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XRCC2 Monoclonal antibody, PBS Only

XRCC2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66652-1-PBS
Clone No.3D9A1

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

X-ray repair cross-complementing protein 2, DNA repair protein XRCC2, 3D9A1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66652-1-PBS targets XRCC2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC2 fusion protein Ag14421 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 2
Calculated molecular weight 280 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC042137
Gene Symbol XRCC2
Gene ID (NCBI) 7516
RRIDAB_2882009
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43543
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.