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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XRCC3 Monoclonal antibody

XRCC3 Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3A12F9

Cat no : 67222-1-Ig

Synonyms

DNA repair protein XRCC3, XRCC3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XRCC3 Monoclonal antibody (67222-1-Ig)

    WB analysis using 67222-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using XRCC3 Monoclonal antibody (67222-1-Ig)

    WB analysis of HT-29 using 67222-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using XRCC3 Monoclonal antibody (67222-1-Ig)

    WB analysis of Caco-2 using 67222-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using XRCC3 Monoclonal antibody (67222-1-Ig)

    WB analysis of HT-1080 using 67222-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 67222-1-Ig (XRCC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using XRCC3 Monoclonal antibody (67222-1-Ig)

    IP experiment of MCF-7 using 67222-1-Ig

    IP result of anti-XRCC3 (IP:67222-1-Ig, 5ug; Detection:67222-1-Ig 1:500) with MCF-7 cells lysate 640 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IHC staining of mouse testis using 67222-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IHC staining of human placenta using 67222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IHC staining of human placenta using 67222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IHC staining of mouse testis using 67222-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67222-1-Ig (XRCC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IF Staining of mouse testis using 67222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using XRCC3 antibody (67222-1-Ig, Clone: 3A12F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using XRCC3 Monoclonal antibody (67222-1-Ig)

    IF Staining of mouse testis using 67222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using XRCC3 antibody (67222-1-Ig, Clone: 3A12F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inNCI-H1299 cells, HT-29 cells, Caco-2 cells, HT-1080 cells, HeLa cells, MCF-7 cells, COLO 320 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67222-1-Ig targets XRCC3 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC3 fusion protein Ag13317 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 3
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC011725
Gene symbol XRCC3
Gene ID (NCBI) 7517
RRIDAB_2882513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for XRCC3 antibody 67222-1-IgDownload protocol
IHC protocol for XRCC3 antibody 67222-1-IgDownload protocol
IF protocol for XRCC3 antibody 67222-1-IgDownload protocol
IP protocol for XRCC3 antibody 67222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols