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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XRCC5 Monoclonal antibody, PBS Only

XRCC5 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G5E7

Cat no : 66546-1-PBS

Synonyms

86 kDa subunit of Ku antigen, CTC85, CTCBF, DNA repair protein XRCC5, G22P2, KARP 1, KARP1, KU80, Ku86, KUB2, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, TLAA, XRCC5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XRCC5 Monoclonal antibody (66546-1-Ig)

    WB analysis using 66546-1-Ig (same clone as 66546-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66546-1-Ig (XRCC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using XRCC5 Monoclonal antibody (66546-1-Ig)

    IP experiment of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    IP result of anti-XRCC5 (IP:66546-1-Ig, 5ug; Detection:66546-1-Ig 1:20000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using XRCC5 Monoclonal antibody (66546-1-Ig)

    IP experiment of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    IP result of anti-XRCC5 (IP:66546-1-Ig, 5ug; Detection:66546-1-Ig 1:40000) with HeLa cells lysate 640 ug. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using XRCC5 Monoclonal antibody (66546-1-Ig)

    IHC staining of human lung cancer using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRCC5 Monoclonal antibody (66546-1-Ig)

    IHC staining of human breast cancer using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRCC5 Monoclonal antibody (66546-1-Ig)

    IHC staining of human breast cancer using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using XRCC5 Monoclonal antibody (66546-1-Ig)

    IHC staining of human lung cancer using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using XRCC5 Monoclonal antibody (66546-1-Ig)

    IF Staining of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using XRCC5 antibody (66546-1-Ig, Clone: 2G5E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using XRCC5 Monoclonal antibody (66546-1-Ig)

    IF Staining of HeLa using 66546-1-Ig (same clone as 66546-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66546-1-Ig (XRCC5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66546-1-PBS targets XRCC5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen XRCC5 fusion protein Ag9512 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Calculated molecular weight 732 aa, 83 kDa
Observed molecular weight 80-83 kDa
GenBank accession numberBC019027
Gene symbol XRCC5
Gene ID (NCBI) 7520
RRIDAB_2881908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.