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KD/KO Validated

XRN2 Polyclonal antibody

Name: XRN2 Polyclonal antibody
Brand: Proteintech
SKU: 11267-1-AP

XRN2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11267-1-AP

Synonyms

5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, DHM1 like protein, DHP protein, XRN2

Validation Data Gallery

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AllWB

WB analysis using 11267-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11267-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11267-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 11267-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11267-1-AP (XRN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Cat No. 11267-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in

HEK-293T cells, COLO 320 cells, HEK-293 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

Product Information

11267-1-AP targets XRN2 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	XRN2 fusion protein Ag1788 相同性解析による交差性が予測される生物種
Full Name	5'-3' exoribonuclease 2
Calculated molecular weight	104 kDa
Observed molecular weight	104 kDa
GenBank accession number	BC006417
Gene symbol	XRN2
Gene ID (NCBI)	22803
RRID	AB_2288787
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.

Protocols

Product Specific Protocols
WB protocol for XRN2 antibody 11267-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article
human	WB	Cell Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII. Authors - Wagschal Alexandre A KD Validated View Article
human	ChIP,CoIP,WB	EMBO J Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids. Authors - Sofiane Y Mersaoui KD Validated View Article
		J Cell Biol Intronless mRNAs transit through nuclear speckles to gain export competence. Authors - Ke Wang View Article
human	WB	PLoS Pathog Characterization of miR-122-independent propagation of HCV. Authors - Chikako Ono KD Validated View Article
human	WB, IF	J Virol Dissecting the roles of the 5' exoribonucleases xrn1 and xrn2 in restricting hepatitis C virus replication. Authors - You Li KD Validated View Article

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Conjugate

Conjugate

XRN2 Polyclonal antibody

XRN2 Polyclonal Antibody for WB, ELISA

Validation Data Gallery

WB analysis using 11267-1-AP

WB analysis of HEK-293 using 11267-1-AP

WB analysis of HeLa using 11267-1-AP

WB analysis of COLO 320 using 11267-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII.

Arginine methylation of the DDX5 helicase RGG/RG motif by PRMT5 regulates resolution of RNA:DNA hybrids.

Intronless mRNAs transit through nuclear speckles to gain export competence.

Characterization of miR-122-independent propagation of HCV.

Dissecting the roles of the 5' exoribonucleases xrn1 and xrn2 in restricting hepatitis C virus replication.