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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XRN2 Monoclonal antibody (66852-1-Ig)

    WB analysis using 66852-1-Ig (same clone as 66852-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66852-1-Ig (XRN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using XRN2 Monoclonal antibody (66852-1-Ig)

    WB analysis of HEK-293 using 66852-1-Ig (same clone as 66852-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66852-1-Ig (XRN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using XRN2 Monoclonal antibody (66852-1-Ig)

    WB analysis of COLO 320 using 66852-1-Ig (same clone as 66852-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66852-1-Ig (XRN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRN2 Monoclonal antibody (66852-1-Ig)

    IHC staining of human breast cancer using 66852-1-Ig (same clone as 66852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66852-1-Ig (XRN2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRN2 Monoclonal antibody (66852-1-Ig)

    IHC staining of human breast cancer using 66852-1-Ig (same clone as 66852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66852-1-Ig (XRN2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using XRN2 Monoclonal antibody (66852-1-Ig)

    IF Staining of MCF-7 using 66852-1-Ig (same clone as 66852-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using XRN2 antibody (66852-1-Ig, Clone: 2C3E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using XRN2 Monoclonal antibody (66852-1-Ig)

    FC experiment of HepG2 using 66852-1-Ig (same clone as 66852-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XRN2 (66852-1-Ig, Clone:2C3E3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66852-1-PBS in a different storage buffer formulation.

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XRN2 Monoclonal antibody, PBS Only

XRN2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66852-1-PBS
Clone No.2C3E3

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66852-1-PBS targets XRN2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen XRN2 fusion protein Ag27927 相同性解析による交差性が予測される生物種
Full Name 5'-3' exoribonuclease 2
Calculated molecular weight 104 kDa
Observed molecular weight 109 kDa
GenBank accession numberBC006417
Gene Symbol XRN2
Gene ID (NCBI) 22803
RRIDAB_2882192
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0D6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.