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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XYLT1 Polyclonal antibody

XYLT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55061-1-AP

Synonyms

Peptide O xylosyltransferase 1, XT I, XT1, Xylosyltransferase 1, xylosyltransferase I, XylT I, XYLT1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using XYLT1 Polyclonal antibody (55061-1-AP)

    WB analysis of HEK-293 using 55061-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using XYLT1 Polyclonal antibody (55061-1-AP)

    WB analysis of BxPC-3 using 55061-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55061-1-AP (XYLT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using XYLT1 Polyclonal antibody (55061-1-AP)

    IHC staining of mouse kidney using 55061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using XYLT1 Polyclonal antibody (55061-1-AP)

    IHC staining of mouse kidney using 55061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using XYLT1 Polyclonal antibody (55061-1-AP)

    IHC staining of mouse pancreas using 55061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using XYLT1 Polyclonal antibody (55061-1-AP)

    IHC staining of mouse pancreas using 55061-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 55061-1-AP (XYLT1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, BxPC-3 cells
Positive IHC detected inmouse kidney tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

55061-1-AP targets XYLT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name xylosyltransferase I
Calculated molecular weight 108 kDa
Observed molecular weight 120 kDa
GenBank accession numberNM_022166
Gene symbol XYLT1
Gene ID (NCBI) 64131
RRIDAB_10804758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XYLT1, also named as XT1, Belongs to the glycosyltransferase 14 family and XylT subfamily. It catalyzes the first step in biosynthesis of glycosaminoglycan. XYLT1 transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. The antibody is specific to XYLT1.

Protocols

Product Specific Protocols
WB protocol for XYLT1 antibody 55061-1-APDownload protocol
IHC protocol for XYLT1 antibody 55061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Phytomedicine

Gua Lou Er Chen decoction attenuates atherosclerosis by reducing proteoglycans accumulation and inflammation

Authors - Hongya Guo
View Article
humanWB

J Biol Chem

Chondroitin sulfate modification of CSPG4 regulates the maintenance and differentiation of glioma initiating cells via integrin-associated signaling

Authors - Akiko Niibori-Nambu
View Article