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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using YAF2 Polyclonal antibody (13980-1-AP)

    WB analysis of HEK-293 using 13980-1-AP

    WB result of YAF2 antibody (13980-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-YAF2 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using YAF2 Polyclonal antibody (13980-1-AP)

    IP experiment of HeLa using 13980-1-AP

    IP result of anti-YAF2 (IP:13980-1-AP, 4ug; Detection:13980-1-AP 1:600) with HeLa cells lysate 1760 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using YAF2 Polyclonal antibody (13980-1-AP)

    IHC staining of mouse brain using 13980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13980-1-AP (YAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using YAF2 Polyclonal antibody (13980-1-AP)

    IHC staining of mouse brain using 13980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13980-1-AP (YAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using YAF2 Polyclonal antibody (13980-1-AP)

    IF Staining of U2OS using 13980-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YAF2 antibody (13980-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YAF2 Polyclonal antibody (13980-1-AP)

    IF Staining of HeLa using 13980-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using YAF2 antibody (13980-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  • Featured Product
  • KD/KO Validated

YAF2 Polyclonal antibody

YAF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13980-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

YY1-associated factor 2, YY1 associated factor 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13980-1-AP targets YAF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YAF2 fusion protein Ag5023 相同性解析による交差性が予測される生物種
Full Name YY1 associated factor 2
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight20-30 kDa
GenBank accession numberBC037777
Gene Symbol YAF2
Gene ID (NCBI) 10138
RRIDAB_3669173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).

Protocols

Product Specific Protocols
WB protocol for YAF2 antibody 13980-1-APDownload protocol
IHC protocol for YAF2 antibody 13980-1-APDownload protocol
IF protocol for YAF2 antibody 13980-1-APDownload protocol
IP protocol for YAF2 antibody 13980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols