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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-YAP1 (Ser127) Recombinant antibody, PBS Only

Phospho-YAP1 (Ser127) Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240779G4

Cat no : 80694-2-PBS

Synonyms

Phospho YAP1, p YAP1 Ser127, p YAP1 (Ser127), p YAP1, Phospho YAP1 (Ser127)

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Phospho-YAP1 (Ser127) Recombinant antibody (80694-2-RR)

    WB analysis of HepG2 using 80694-2-RR (same clone as 80694-2-PBS)

    Calyculin A treated and untreated HepG2 cells were subjected to SDS PAGE followed by western blot with 80694-2-RR (Phospho-YAP1 (Ser127) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80694-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Phospho-YAP1 (Ser127) Recombinant antibody (80694-2-RR)

    FC experiment of HepG2 using 80694-2-RR (same clone as 80694-2-PBS)

    1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A intracellularly stained with 0.13 ug Phospho-YAP1 (Ser127) Recombinant antibody (80694-2-RR, Clone:240779G4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80694-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80694-2-PBS targets Phospho-YAP1 (Ser127) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Yes-associated protein 1, 65kDa
Calculated molecular weight 504 aa, 54 kDa
Observed molecular weight70-78 kDa
GenBank accession numberBC038235
Gene symbol YAP1
Gene ID (NCBI) 10413
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)