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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YARS Polyclonal antibody (31228-1-AP)

    WB analysis using 31228-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31228-1-AP (YARS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using YARS Polyclonal antibody (31228-1-AP)

    IHC staining of human ovary cancer using 31228-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using YARS Polyclonal antibody (31228-1-AP)

    IHC staining of human ovary cancer using 31228-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using YARS Polyclonal antibody (31228-1-AP)

    IHC staining of human stomach cancer using 31228-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using YARS Polyclonal antibody (31228-1-AP)

    IHC staining of human stomach cancer using 31228-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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YARS Polyclonal antibody

YARS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31228-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, ELISA

Synonyms

YARS1, YTS, YRS, TYRRS, CMTDIC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, U2OS cells, pig pancreas tissue, pig tonsil tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31228-1-AP targets YARS in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YARS fusion protein Ag35148 相同性解析による交差性が予測される生物種
Full Name tyrosyl-tRNA synthetase
Calculated molecular weight 59 kDa
Observed molecular weight60 kDa
GenBank accession numberBC016689
Gene Symbol YARS
Gene ID (NCBI) 8565
RRIDAB_3669909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP54577
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)

Protocols

Product Specific Protocols
WB protocol for YARS antibody 31228-1-APDownload protocol
IHC protocol for YARS antibody 31228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols