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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YARS Recombinant antibody (84859-3-RR)

    WB analysis using 84859-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84859-3-RR (YARS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using YARS Recombinant antibody (84859-3-RR)

    IHC staining of human ovary cancer using 84859-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using YARS Recombinant antibody (84859-3-RR)

    IHC staining of human ovary cancer using 84859-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using YARS Recombinant antibody (84859-3-RR)

    IF Staining of U2OS using 84859-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YARS antibody (84859-3-RR, Clone: 242400E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84859-3-RR

    Affinity and Kinetic Characterization of 84859-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84859-3-RR against Human YARS were performed. The affinity constant is 0.414 nM.

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YARS Recombinant antibody

YARS Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84859-3-RR
Clone No.242400E9

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

YARS1, YTS, YRS, TYRRS, CMTDIC

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84859-3-RR targets YARS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YARS fusion protein Ag35148 相同性解析による交差性が予測される生物種
Full Name tyrosyl-tRNA synthetase
Calculated molecular weight 59 kDa
Observed molecular weight60 kDa
GenBank accession numberBC016689
Gene Symbol YARS
Gene ID (NCBI) 8565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54577
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)

Protocols

Product Specific Protocols
WB protocol for YARS antibody 84859-3-RRDownload protocol
IHC protocol for YARS antibody 84859-3-RRDownload protocol
IF protocol for YARS antibody 84859-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols