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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YBX1 Recombinant antibody (84993-4-RR)

    WB analysis using 84993-4-RR (same clone as 84993-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84993-4-RR (YBX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84993-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YBX1 Recombinant antibody (84993-4-RR)

    IHC staining of human colon cancer using 84993-4-RR (same clone as 84993-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84993-4-RR (YBX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84993-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YBX1 Recombinant antibody (84993-4-RR)

    IHC staining of human colon cancer using 84993-4-RR (same clone as 84993-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84993-4-RR (YBX1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84993-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YBX1 Recombinant antibody (84993-4-RR)

    IF Staining of HeLa using 84993-4-RR (same clone as 84993-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using YBX1 antibody (84993-4-RR, Clone: 242462F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84993-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using YBX1 Recombinant antibody (84993-4-RR)

    IF Staining of Neuro-2a using 84993-4-RR (same clone as 84993-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using YBX1 antibody (84993-4-RR, Clone: 242462F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84993-4-PBS in a different storage buffer formulation.

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YBX1 Recombinant antibody, PBS Only

YBX1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84993-4-PBS
Clone No.242462F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242462F12, CBF A, CBF-A, CCAAT-binding transcription factor I subunit A, DBPB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84993-4-PBS targets YBX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YBX1 fusion protein Ag14129 相同性解析による交差性が予測される生物種
Full Name Y box binding protein 1
Calculated molecular weight 324 aa, 36 kDa
Observed molecular weight 36-56 kDa
GenBank accession numberBC010430
Gene Symbol YBX1
Gene ID (NCBI) 4904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP67809
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Y box binding protein 1(YBX1) is one of cis-acting elements which has a role in regulation of the expression of HLA class II genes. YBX1 is great of importance in regulation of PTP1B expression. It can bind to splice sites mediate pre-mRNA alternative splicing regulation. Also, it involves the regulation of translation by modulation of the interaction between the mRNA and eukaryotic initiation factors. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. The calculated molecular weight of YBX1 is 36 kDa, but modified YBX1 is about 36-56 kDa. (PMID: 27384875 )