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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

YES1 Polyclonal antibody

YES1 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13977-1-AP

Synonyms

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of A431 cells using YES1 Polyclonal antibody (13977-1-AP)

    IP experiment of A431 using 13977-1-AP

    IP result of anti-YES1 (IP:13977-1-AP, 4ug; Detection:13977-1-AP 1:300) with A431 cells lysate 2280 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YES1 Polyclonal antibody (13977-1-AP)

    IHC staining of human breast cancer using 13977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13977-1-AP (YES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YES1 Polyclonal antibody (13977-1-AP)

    IHC staining of human breast cancer using 13977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13977-1-AP (YES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YES1 Polyclonal antibody (13977-1-AP)

    IHC staining of human colon cancer using 13977-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13977-1-AP (YES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YES1 Polyclonal antibody (13977-1-AP)

    IHC staining of human colon cancer using 13977-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13977-1-AP (YES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13977-1-AP targets YES1 in IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YES1 fusion protein Ag5016 相同性解析による交差性が予測される生物種
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Calculated molecular weight 61 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC048960
Gene symbol YES1
Gene ID (NCBI) 7525
RRIDAB_2877998
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Protocols

Product Specific Protocols
IHC protocol for YES1 antibody 13977-1-APDownload protocol
IP protocol for YES1 antibody 13977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols