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  • KD/KO Validated

YES1-Specific Polyclonal antibody

YES1-Specific Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20243-1-AP

Synonyms

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1, YES1-Specific


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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, A549 cells, DU 145 cells, HEK-293 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20243-1-AP targets YES1-Specific in IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberNM_005433
Gene symbol YES1
Gene ID (NCBI) 7525
RRIDAB_10697656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody is specific to YES1.

Protocols

Product Specific Protocols
WB protocol for YES1-Specific antibody 20243-1-APDownload protocol
IHC protocol for YES1-Specific antibody 20243-1-APDownload protocol
IP protocol for YES1-Specific antibody 20243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Am J Respir Crit Care Med

YES1 Drives Lung Cancer Growth and Progression and Predicts Sensitivity to Dasatinib.

Authors - Irati Garmendia
human,mouse

Sci Adv

A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.

Authors - Jackson A Roberts
humanIHC

J Immunother Cancer

SRC family kinase (SFK) inhibitor dasatinib improves the antitumor activity of anti-PD-1 in NSCLC models by inhibiting Treg cell conversion and proliferation.

Authors - Esther Redin
  • KO Validated
human

Cell Death Dis

Linc01133 contributes to gastric cancer growth by enhancing YES1-dependent YAP1 nuclear translocation via sponging miR-145-5p.

Authors - Yujing Sun
human

J Cell Biochem

miR-17-5p up-regulates YES1 to modulate the cell cycle progression and apoptosis in ovarian cancer cell lines.

Authors - Lan Li
  • KD Validated
human

iScience

DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer

Authors - Qingwen Huang