• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

YIPF4 Polyclonal antibody

YIPF4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15473-1-AP

Synonyms

FinGER4, Nbla11189, Protein YIPF4, Yip1 domain family, member 4, YIP1 family member 4, YIPF4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using YIPF4 Polyclonal antibody (15473-1-AP)

    WB analysis using 15473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using YIPF4 Polyclonal antibody (15473-1-AP)

    WB analysis using 15473-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using YIPF4 Polyclonal antibody (15473-1-AP)

    IHC staining of human endometrial cancer using 15473-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using YIPF4 Polyclonal antibody (15473-1-AP)

    IHC staining of human endometrial cancer using 15473-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YIPF4 Polyclonal antibody (15473-1-AP)

    IF Staining of HeLa using 15473-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using YIPF4 antibody (15473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YIPF4 Polyclonal antibody (15473-1-AP)

    IF Staining of HeLa using 15473-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15473-1-AP (YIPF4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Red: CL594-phalloidin staining of F-actin.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, Jurkat cells, HaCaT cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15473-1-AP targets YIPF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YIPF4 fusion protein Ag7777 相同性解析による交差性が予測される生物種
Full Name Yip1 domain family, member 4
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC004875
Gene symbol YIPF4
Gene ID (NCBI) 84272
RRIDAB_2217206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for YIPF4 antibody 15473-1-APDownload protocol
IHC protocol for YIPF4 antibody 15473-1-APDownload protocol
IF protocol for YIPF4 antibody 15473-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols