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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

YME1L1 Polyclonal antibody

YME1L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27856-1-AP

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YME1L1 Polyclonal antibody (27856-1-AP)

    WB analysis using 27856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27856-1-AP (YME1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using YME1L1 Polyclonal antibody (27856-1-AP)

    IHC staining of mouse liver using 27856-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27856-1-AP (YME1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YME1L1 Polyclonal antibody (27856-1-AP)

    IF Staining of HeLa using 27856-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using YME1L1 antibody (27856-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27856-1-AP targets YME1L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YME1L1 fusion protein Ag27280 相同性解析による交差性が予測される生物種
Full Name YME1-like 1 (S. cerevisiae)
Calculated molecular weight 716 aa, 80 kDa
Observed molecular weight63 kDa
GenBank accession numberBC023507
Gene symbol YME1L1
Gene ID (NCBI) 10730
RRIDAB_3085999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

Protocols

Product Specific Protocols
WB protocol for YME1L1 antibody 27856-1-APDownload protocol
IHC protocol for YME1L1 antibody 27856-1-APDownload protocol
IF protocol for YME1L1 antibody 27856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols