Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 25370-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 25370-1-AP (YOD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 25370-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 25370-1-AP (YOD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 25370-1-AP
IP result of anti-YOD1 (IP:25370-1-AP, 4ug; Detection:25370-1-AP 1:6000) with K-562 cells lysate 2400 ug.
IHC staining of human spleen using 25370-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25370-1-AP (YOD1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 25370-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25370-1-AP (YOD1 antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

YOD1 Polyclonal antibody

YOD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 25370-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

DUBA 8, DUBA8, HIN 7, HIN7, HIV 1 induced protease 7, HsHIN7, OTUD2, PRO0907, Ubiquitin thioesterase OTU1, YOD1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 5 publications below
IP	See 2 publications below

Product Information

25370-1-AP targets YOD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	YOD1 fusion protein Ag21881 相同性解析による交差性が予測される生物種
Full Name	YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
Calculated molecular weight	348 aa, 38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC137167
Gene Symbol	YOD1
Gene ID (NCBI)	55432
RRID	AB_2880048
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5VVQ6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for YOD1 antibody 25370-1-AP	Download protocol
IHC protocol for YOD1 antibody 25370-1-AP	Download protocol
IP protocol for YOD1 antibody 25370-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Lett POH1 induces Smad3 deubiquitination and promotes lung cancer metastasis Authors - Yang Yuan View Article
mouse	WB	Comput Struct Biotechnol J Identification of potential diagnostic and prognostic biomarkers for sepsis based on machine learning Authors - Li Ke View Article
pig	WB	Mol Cell Proteomics The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators Authors - Nitchakarn Kaokhum View Article
human	WB	Cell Signal Hypoxic tumor-derived exosomal miR-21 induces cancer-associated fibroblast activation to promote head and neck squamous cell carcinoma metastasis Authors - Beibei Ye View Article
human	WB,IHC	Cancer Cell Int YOD1 serves as a potential prognostic biomarker for pancreatic cancer. Authors - Zhishuo Zhang View Article
human	WB	Biomed Res Int Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway. Authors - Xiao-Long Zhou KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 25370-1-AP

WB analysis of K-562 using 25370-1-AP

IP experiment of K-562 using 25370-1-AP

IHC staining of human spleen using 25370-1-AP

IHC staining of human spleen using 25370-1-AP

YOD1 Polyclonal antibody

YOD1 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

POH1 induces Smad3 deubiquitination and promotes lung cancer metastasis

Identification of potential diagnostic and prognostic biomarkers for sepsis based on machine learning

The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators

Hypoxic tumor-derived exosomal miR-21 induces cancer-associated fibroblast activation to promote head and neck squamous cell carcinoma metastasis

YOD1 serves as a potential prognostic biomarker for pancreatic cancer.

Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway.