Validation Data Gallery

Filter: AllWBIHC

WB analysis of HEK-293 using 14392-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14392-1-AP (YTHDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse tissue using 14392-1-AP
WB result of YTHDC1 (14392-1-AP) antibody with mouse sample.
WB analysis of MCF-7 using 14392-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 14392-1-AP (YTHDC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 14392-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 14392-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 14392-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14392-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14392-1-AP (YTHDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

YTHDC1 Polyclonal antibody

YTHDC1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14392-1-AP

Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (42)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, MCF-7 cells, mouse tissue
Positive IHC detected in	mouse brain tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 33 publications below
IHC	See 1 publications below
IF	See 6 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 10 publications below

Product Information

14392-1-AP targets YTHDC1 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	YTHDC1 fusion protein Ag5882 相同性解析による交差性が予測される生物種
Full Name	YTH domain containing 1
Calculated molecular weight	85 kDa
Observed molecular weight	~100 kDa
GenBank accession number	BC053863
Gene Symbol	YTHDC1
Gene ID (NCBI)	91746
RRID	AB_2878052
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96MU7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

YTHDC1, also named as KIAA1966 or YT521, is a 727 animo acid protein, which contains 1 YTH domain. YTHDC1 localizes in the nucleus and Interacts with EMD, KHDRBS1/SAM68, KHDRBS3, TRA2B/SFRS10, SC35 and SF2. YTHDC1 as a RNA-binding protein that regulates alternative splice site selection. The calcualted molecular weight of YTHDC1 is 85 kDa, but modified TFEB is about 110 kDa (PMID: 10973987 ).

Protocols

Product Specific Protocols
WB protocol for YTHDC1 antibody 14392-1-AP	Download protocol
IHC protocol for YTHDC1 antibody 14392-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature m6A facilitates hippocampus-dependent learning and memory through YTHDF1. Authors - Hailing Shi View Article
human	WB	Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis. Authors - Shujie Chen View Article
human	WB,IF	Nucleic Acids Res N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication. Authors - Haojie Hao View Article
human	WB	Cell Death Differ YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect. Authors - Yichao Hou View Article
mouse	RIP	Dev Cell N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation. Authors - Jie Du View Article
human	WB	J Neuroinflammation m6A-methylase METTL3 promotes retinal angiogenesis through modulation of metabolic reprogramming in RPE cells Authors - Qian Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 14392-1-AP

WB analysis of mouse tissue using 14392-1-AP

WB analysis of MCF-7 using 14392-1-AP

IHC staining of mouse brain using 14392-1-AP

IHC staining of human ovary tumor using 14392-1-AP

IHC staining of human ovary tumor using 14392-1-AP

IHC staining of mouse brain using 14392-1-AP

YTHDC1 Polyclonal antibody

YTHDC1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.

YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect.

N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.

m6A-methylase METTL3 promotes retinal angiogenesis through modulation of metabolic reprogramming in RPE cells