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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

YTHDC1 Monoclonal antibody, PBS Only

YTHDC1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F5G11

Cat no : 67911-1-PBS

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YTHDC1 Monoclonal antibody (67911-1-Ig)

    WB analysis using 67911-1-Ig (same clone as 67911-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67911-1-Ig (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YTHDC1 Monoclonal antibody (67911-1-Ig)

    IHC staining of human breast cancer using 67911-1-Ig (same clone as 67911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YTHDC1 Monoclonal antibody (67911-1-Ig)

    IHC staining of human breast cancer using 67911-1-Ig (same clone as 67911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using YTHDC1 Monoclonal antibody (67911-1-Ig)

    IHC staining of human cervical cancer using 67911-1-Ig (same clone as 67911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using YTHDC1 Monoclonal antibody (67911-1-Ig)

    IHC staining of mouse testis using 67911-1-Ig (same clone as 67911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using YTHDC1 Monoclonal antibody (67911-1-Ig)

    IHC staining of mouse testis using 67911-1-Ig (same clone as 67911-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67911-1-PBS targets YTHDC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen YTHDC1 fusion protein Ag6289 相同性解析による交差性が予測される生物種
Full Name YTH domain containing 1
Calculated molecular weight 85 kDa
Observed molecular weight~100 kDa
GenBank accession numberBC053863
Gene symbol YTHDC1
Gene ID (NCBI) 91746
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).