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  • KD/KO Validated

YTHDC2 Polyclonal antibody

YTHDC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 27779-1-AP

Synonyms

YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2


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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inrat testis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27779-1-AP targets YTHDC2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDC2 fusion protein Ag26690 相同性解析による交差性が予測される生物種
Full Name YTH domain containing 2
Observed molecular weight 160 kDa
GenBank accession numberBC137285
Gene symbol YTHDC2
Gene ID (NCBI) 64848
RRIDAB_2880970
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

Protocols

Product Specific Protocols
WB protocol for YTHDC2 antibody 27779-1-APDownload protocol
IHC protocol for YTHDC2 antibody 27779-1-APDownload protocol
IF protocol for YTHDC2 antibody 27779-1-APDownload protocol
FC protocol for YTHDC2 antibody 27779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

Inhibition of METTL3 in macrophages provides protection against intestinal inflammation

Authors - Huilong Yin
humanIF

Mol Cell

Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.

Authors - Hagai Marmor-Kollet
humanWB

Sci Adv

Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A).

Authors - Ji Hoon Lee
humanWB

Nat Commun

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

Authors - Shujie Chen
humanWB,RIP

Cancer Lett

LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation

Authors - Lu Chen
  • KD Validated
mouseWB,IP

Genes Dev

YTHDC2 control of gametogenesis requires helicase activity but not m6A binding.

Authors - Yuhki Saito

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maialen (Verified Customer) (02-10-2022)

YTHDC2 was immunoprecipitated from 4 million HCT15 cells lysate using 1μg 27779-1-AP (YTHDC2 antibody). Same amount of normal rabbit IgG was used as negative control. For IP confirmation, western blot with 27779-1-AP (YTHDC2 antibody) was perfomed at 1:1000 dilution incubated overnight at 4ºC. 10% of the whole cell lysate was loaded as input.

  • Applications: Western Blot, Immunoprecipitation
  • Cell Tissue Type: HCT15
YTHDC2 Antibody Western Blot,Immunoprecipitation validation ( dilution) in HCT15 (Cat no:27779-1-AP)
FH

Robert (Verified Customer) (01-25-2019)

1. scrambled siRNA2. YTHDC2 siRNA12. YTHDC2 siRNA22. YTHDC2 siRNA3

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: ST cells (porcine)
YTHDC2 Antibody Western Blot, validation (1/500 dilution) in ST cells (porcine) (Cat no:27779-1-AP)