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  • Featured Product
  • KD/KO Validated

YTHDC2 Polyclonal antibody, PBS Only

YTHDC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 27779-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YTHDC2 Polyclonal antibody (27779-1-AP)

    WB analysis using 27779-1-AP (same clone as 27779-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27779-1-AP (YTHDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using YTHDC2 Polyclonal antibody (27779-1-AP)

    WB analysis of HepG2 using 27779-1-AP (same clone as 27779-1-PBS)

    WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HepG2 cells. This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using YTHDC2 Polyclonal antibody (27779-1-AP)

    WB analysis of HeLa using 27779-1-AP (same clone as 27779-1-PBS)

    WB result of YTHDC2 antibody (27779-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HeLa cells. This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using YTHDC2 Polyclonal antibody (27779-1-AP)

    IHC staining of rat testis using 27779-1-AP (same clone as 27779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using YTHDC2 Polyclonal antibody (27779-1-AP)

    IHC staining of rat testis using 27779-1-AP (same clone as 27779-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 27779-1-AP (YTHDC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YTHDC2 Polyclonal antibody (27779-1-AP)

    IF Staining of HepG2 using 27779-1-AP (same clone as 27779-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using YTHDC2 antibody (27779-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using YTHDC2 Polyclonal antibody (27779-1-AP)

    FC experiment of HeLa using 27779-1-AP (same clone as 27779-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human YTHDC2 (27779-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27779-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27779-1-PBS targets YTHDC2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDC2 fusion protein Ag26690 相同性解析による交差性が予測される生物種
Full Name YTH domain containing 2
Observed molecular weight 160 kDa
GenBank accession numberBC137285
Gene symbol YTHDC2
Gene ID (NCBI) 64848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.