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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

YTHDC2 Recombinant antibody, PBS Only (Detector)

YTHDC2 Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241123A9

Cat no : 83970-1-PBS

Synonyms

YTH domain-containing protein 2, hYTHDC2, EC:3.6.4.13, 3'-5' RNA helicase YTHDC2, 241123A9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YTHDC2 Recombinant antibody (83970-1-RR)

    WB analysis using 83970-1-RR (same clone as 83970-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83970-1-RR (YTHDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00893-3

    Sandwich ELISA standard curve of MP00893-3

    Sandwich ELISA standard curve of MP00893-3, Human YTHDC2 Recombinant Matched Antibody Pair - PBS only. 83970-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26690. 83970-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL

  • Sample test of MP00893-3

    Sample test of MP00893-3

    The mean YTHDC2 concentration was determined to be 4.89 ng/mL in HEK-293T cell extract based on a 1.4 mg/mL extract load.

  • Immunohistochemistry (IHC) staining of rat testis tissue using YTHDC2 Recombinant antibody (83970-1-RR)

    IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using YTHDC2 Recombinant antibody (83970-1-RR)

    IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using YTHDC2 Recombinant antibody (83970-1-RR)

    FC experiment of HepG2 using 83970-1-RR (same clone as 83970-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00893-1

    Cytometric bead array standard curve of MP00893-1

    Cytometric bead array standard curve of MP00893-1, YTHDC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83970-4-PBS. Detection antibody: 83970-1-PBS. Standard: Ag26690. Range: 0.156-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83970-1-PBS targets YTHDC2 as part of a matched antibody pair:

MP00893-1: 83970-4-PBS capture and 83970-1-PBS detection (validated in Cytometric bead array)

MP00893-3: 83970-3-PBS capture and 83970-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YTHDC2 fusion protein Ag26690 相同性解析による交差性が予測される生物種
Full Name YTH domain containing 2
Observed molecular weight160 kDa
GenBank accession numberBC137285
Gene symbol YTHDC2
Gene ID (NCBI) 64848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.