YTHDF1 Polyclonal antibody
YTHDF1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 26787-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| RIP | See 1 publications below |
Product Information
The immunogen of 26787-1-AP is YTHDF1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | YTHDF1 fusion protein Ag25347 相同性解析による交差性が予測される生物種 |
| Full Name | YTH domain family, member 1 |
| Calculated molecular weight | 559 aa, 61 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC050284 |
| Gene symbol | YTHDF1 |
| Gene ID (NCBI) | 54915 |
| RRID | AB_2880635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDF1 antibody 26787-1-AP | Download protocol |
| IP protocol for YTHDF1 antibody 26787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis | ||
Cell Mol Neurobiol Wnt3a/YTHDF1 Regulated Oxaliplatin-Induced Neuropathic Pain Via TNF-α/IL-18 Expression in the Spinal Cord | ||
Cancer Med BIRC5 facilitates cisplatin-chemoresistance in a m6 A-dependent manner in ovarian cancer | ||
Cancer Cell Int DUSP5 regulated by YTHDF1-mediated m6A modification promotes epithelial-mesenchymal transition and EGFR-TKI resistance via the TGF-β/Smad signaling pathway in lung adenocarcinoma
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