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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using YTHDF1 Monoclonal antibody (66745-1-Ig)

    WB analysis of HepG2 using 66745-1-Ig (same clone as 66745-1-PBS)

    WB result of YTHDF1 antibody (66745-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF1 transfected HepG2 cells. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using YTHDF1 Monoclonal antibody (66745-1-Ig)

    WB analysis using 66745-1-Ig (same clone as 66745-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using YTHDF1 Monoclonal antibody (66745-1-Ig)

    WB analysis using 66745-1-Ig (same clone as 66745-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IP experiment of Jurkat using 66745-1-Ig (same clone as 66745-1-PBS)

    IP result of anti-YTHDF1 (IP:66745-1-Ig, 4ug; Detection:66745-1-Ig 1:2000) with Jurkat cells lysate 2000 ug. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of human breast cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of human breast cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of mouse testis using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of mouse testis using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of human lung cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of human lung cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of rat testis using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of rat testis using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using YTHDF1 Monoclonal antibody (66745-1-Ig)

    IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

YTHDF1 Monoclonal antibody, PBS Only

YTHDF1 Monoclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 66745-1-PBS
Clone No.3A2H12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

YTH domain-containing family protein 1, DF1, DACA-1, C20orf21, 3A2H12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66745-1-PBS targets YTHDF1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen YTHDF1 fusion protein Ag11633 相同性解析による交差性が予測される生物種
Full Name YTH domain family, member 1
Calculated molecular weight 559 aa, 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC050284
Gene Symbol YTHDF1
Gene ID (NCBI) 54915
RRIDAB_2882093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BYJ9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.