Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, Jurkat cells, HeLa cells, MOLT-4 cells, NIH/3T3 cells, C6 cells, Raji cells |
Positive IP detected in | HeLa cells |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | U2OS cells, HeLa cells, sodium arsenite treated HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
24744-1-AP targets YTHDF2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, chIP, RIP, ELISA, CLIP applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, monkey, chicken, sheep, goat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | YTHDF2 fusion protein Ag5922 相同性解析による交差性が予測される生物種 |
Full Name | YTH domain family, member 2 |
Calculated molecular weight | 62 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC002559 |
Gene Symbol | YTHDF2 |
Gene ID (NCBI) | 51441 |
RRID | AB_2687435 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y5A9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
IF protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
IP protocol for YTHDF2 antibody 24744-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature Dynamic m(6)A mRNA methylation directs translational control of heat shock response. | ||
Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. | ||
Nat Med Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy. |