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  • Featured Product
  • KD/KO Validated

YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, chIP, RIP, ELISA, CLIP

Conjugate

Unconjugated

Cat no : 24744-1-AP

Synonyms

YTH domain family protein 2, NY REN 2, HGRG8, DF2, CLL-associated antigen KW-14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YTHDF2 Polyclonal antibody (24744-1-AP)

    WB analysis using 24744-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using YTHDF2 Polyclonal antibody (24744-1-AP)

    WB analysis of Jurkat using 24744-1-AP

    WB result of YTHDF2 antibody (24744-1-AP, 1:4000) with si-control and si-YTHDF2 transfected Jurkat cells..

  • Western Blot (WB) analysis of various lysates using YTHDF2 Polyclonal antibody (24744-1-AP)

    WB analysis using 24744-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using YTHDF2 Polyclonal antibody (24744-1-AP)

    IP experiment of HeLa using 24744-1-AP

    IP result of anti-YTHDF2 (IP:24744-1-AP, 4ug; Detection:24744-1-AP 1:6000) with HeLa cells lysate 1360 ug.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using YTHDF2 Polyclonal antibody (24744-1-AP)

    IF Staining of mouse brain using 24744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using YTHDF2 Polyclonal antibody (24744-1-AP)

    IF Staining of U2OS using 24744-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YTHDF2 Polyclonal antibody (24744-1-AP)

    IF Staining of HeLa using 24744-1-AP

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using YTHDF2 Polyclonal antibody (24744-1-AP)

    IF Staining of U2OS using 24744-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YTHDF2 Polyclonal antibody (24744-1-AP)

    IF Staining of HeLa using 24744-1-AP

    Immunofluorescent analysis of HeLa cells using 24744-1-AP (YTHDF2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, MOLT-4 cells, NIH/3T3 cells, C6 cells, Raji cells
Positive IP detected inHeLa cells
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inU2OS cells, HeLa cells, sodium arsenite treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24744-1-AP targets YTHDF2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, chIP, RIP, ELISA, CLIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey, chicken, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF2 fusion protein Ag5922 相同性解析による交差性が予測される生物種
Full Name YTH domain family, member 2
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC002559
Gene symbol YTHDF2
Gene ID (NCBI) 51441
RRIDAB_2687435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

Protocols

Product Specific Protocols
WB protocol for YTHDF2 antibody 24744-1-APDownload protocol
IF protocol for YTHDF2 antibody 24744-1-APDownload protocol
IP protocol for YTHDF2 antibody 24744-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

m6A enhances the phase separation potential of mRNA.

Authors - Ryan J Ries
View Article
mouseRIP

Nature

m6A RNA methylation regulates the fate of endogenous retroviruses.

Authors - Tomasz Chelmicki
  • KO Validated
View Article
mouseWB

Nature

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi
View Article
human, mouseWB, IF

Nature

Dynamic m(6)A mRNA methylation directs translational control of heat shock response.

Authors - Jun Zhou
View Article
humanWB

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang
View Article
human,mouseFC

Nat Med

Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.

Authors - Irene Ganan-Gomez
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tatyana (Verified Customer) (12-22-2024)

Works well in WB, ICC and IHC. ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Cytoplasmic staining, recognises stress granules. WB with antibody diluted in 4% milk in TBST, with ON incubation. Also gives what looks like specific staining in mouse spinal cord (DAB and fluorescence).

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa and SH-SY5Y cells/human; mouse tissue
YTHDF2 Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in HeLa and SH-SY5Y cells/human; mouse tissue (Cat no:24744-1-AP)
FH

Jinyun (Verified Customer) (08-26-2022)

I did the western blot, and the antibody worked quite well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
FH

Robert (Verified Customer) (01-25-2019)

1. scrambled siRNA2. YTHDF2 siRNA

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
YTHDF2 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:24744-1-AP)