Validation Data Gallery

Filter: AllWBIPIF-PIF/ICC

WB analysis of Jurkat using 24744-1-AP
WB result of YTHDF2 antibody (24744-1-AP, 1:4000) with si-control and si-YTHDF2 transfected Jurkat cells..
WB analysis using 24744-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 24744-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24744-1-AP (YTHDF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 24744-1-AP
IP result of anti-YTHDF2 (IP:24744-1-AP, 4ug; Detection:24744-1-AP 1:6000) with HeLa cells lysate 1360 ug.
IF Staining of mouse brain using 24744-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 24744-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 24744-1-AP
Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).
IF Staining of U2OS using 24744-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using YTHDF2 antibody (24744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 24744-1-AP
Immunofluorescent analysis of HeLa cells using 24744-1-AP (YTHDF2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Featured Product
KD/KO Validated

YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Cat No. 24744-1-AP

Publications(310)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, chIP, RIP, ELISA, CLIP

Synonyms

YTH domain family protein 2, NY REN 2, HGRG8, DF2, CLL-associated antigen KW-14

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (310)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, HeLa cells, MOLT-4 cells, NIH/3T3 cells, C6 cells, Raji cells
Positive IP detected in	HeLa cells
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	U2OS cells, HeLa cells, sodium arsenite treated HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 81 publications below
WB	See 255 publications below
IHC	See 21 publications below
IF	See 43 publications below
IP	See 18 publications below
FC	See 1 publications below
CoIP	See 8 publications below
ChIP	See 1 publications below
RIP	See 63 publications below

Product Information

24744-1-AP targets YTHDF2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, chIP, RIP, ELISA, CLIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	YTHDF2 fusion protein Ag5922 相同性解析による交差性が予測される生物種
Full Name	YTH domain family, member 2
Calculated molecular weight	62 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC002559
Gene Symbol	YTHDF2
Gene ID (NCBI)	51441
RRID	AB_2687435
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y5A9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.

Protocols

Product Specific Protocols
WB protocol for YTHDF2 antibody 24744-1-AP	Download protocol
IF protocol for YTHDF2 antibody 24744-1-AP	Download protocol
IP protocol for YTHDF2 antibody 24744-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nature m6A enhances the phase separation potential of mRNA. Authors - Ryan J Ries View Article
mouse	RIP	Nature m6A RNA methylation regulates the fate of endogenous retroviruses. Authors - Tomasz Chelmicki KO Validated View Article
mouse	WB	Nature m6A facilitates hippocampus-dependent learning and memory through YTHDF1. Authors - Hailing Shi View Article
human, mouse	WB, IF	Nature Dynamic m(6)A mRNA methylation directs translational control of heat shock response. Authors - Jun Zhou View Article
human	WB	Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. Authors - Peiguo Yang View Article
human,mouse	FC	Nat Med Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy. Authors - Irene Ganan-Gomez View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 24744-1-AP

WB analysis using 24744-1-AP

WB analysis using 24744-1-AP

IP experiment of HeLa using 24744-1-AP

IF Staining of mouse brain using 24744-1-AP

IF Staining of U2OS using 24744-1-AP

IF Staining of HeLa using 24744-1-AP

IF Staining of U2OS using 24744-1-AP

IF Staining of HeLa using 24744-1-AP

YTHDF2 Polyclonal antibody

YTHDF2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

m6A enhances the phase separation potential of mRNA.

m6A RNA methylation regulates the fate of endogenous retroviruses.

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Dynamic m(6)A mRNA methylation directs translational control of heat shock response.

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.