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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YTHDF3 Recombinant antibody (84709-3-RR)

    WB analysis using 84709-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84709-3-RR (YTHDF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84709-3-RR

    Affinity and Kinetic Characterization of 84709-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84709-3-RR against Human YTHDF3 were performed. The affinity constant is 0.18 nM.

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YTHDF3 Recombinant antibody

YTHDF3 Recombinant Antibody for WB, ELISA
Cat No. 84709-3-RR
Clone No.242149F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

YTH domain-containing family protein 3, DF3, 242149F3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, PC-12 cells, C6 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84709-3-RR targets YTHDF3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YTHDF3 fusion protein Ag22407 相同性解析による交差性が予測される生物種
Full Name YTH domain family, member 3
Calculated molecular weight 585 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC052970
Gene Symbol YTHDF3
Gene ID (NCBI) 253943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z739
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

Protocols

Product Specific Protocols
WB protocol for YTHDF3 antibody 84709-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols