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- KD/KO Validated
YY1 Polyclonal antibody
YY1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 22156-1-AP
Synonyms
"YY1 Antibodies" Comparison
View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, A549 cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 26 publications below |
| IHC | See 3 publications below |
| IF | See 7 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
| ChIP | See 11 publications below |
Product Information
22156-1-AP targets YY1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | YY1 fusion protein Ag17792 相同性解析による交差性が予測される生物種 |
| Full Name | YY1 transcription factor |
| Calculated molecular weight | 414 aa, 45 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC037308 |
| Gene symbol | YY1 |
| Gene ID (NCBI) | 7528 |
| RRID | AB_11182932 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YY1 antibody 22156-1-AP | Download protocol |
| IHC protocol for YY1 antibody 22156-1-AP | Download protocol |
| IF protocol for YY1 antibody 22156-1-AP | Download protocol |
| IP protocol for YY1 antibody 22156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Transl Med Yin Yang 1 protein ameliorates diabetic nephropathy pathology through transcriptional repression of TGFβ1. | ||
Mol Cell Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis. | ||
Theranostics KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.
| ||
Cancer Lett MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma. | ||
Free Radic Biol Med Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation. | ||
Cancers (Basel) HPV8 Reverses the Transcriptional Output in Lrig1 Positive Cells to Drive Skin Tumorigenesis. |
