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  • KD/KO Validated

YY1 Polyclonal antibody

YY1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 22156-1-AP

Synonyms

UCRBP, NF-E1, INO80S, INO 80S, Delta transcription factor


"YY1 Antibodies" Comparison

View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
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  • Western Blot (WB) analysis of Jurkat cells using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis of Jurkat using 22156-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis of A549 cells using 22156-1-AP

    A549 cells (shcontrol and shRNA of YY1) were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000.

  • Western Blot (WB) analysis of various lysates using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis using 22156-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis of HeLa using 22156-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis of HEK-293 using 22156-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using YY1 Polyclonal antibody (22156-1-AP)

    IP experiment of HeLa using 22156-1-AP

    IP result of anti-YY1 (IP:22156-1-AP, 5ug; Detection:22156-1-AP 1:700) with HeLa cells lysate 880ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YY1 Polyclonal antibody (22156-1-AP)

    IHC staining of human breast cancer using 22156-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YY1 Polyclonal antibody (22156-1-AP)

    IHC staining of human breast cancer using 22156-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Polyclonal antibody (22156-1-AP)

    IF Staining of HepG2 using 22156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (22156-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, A549 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22156-1-AP targets YY1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YY1 fusion protein Ag17792 相同性解析による交差性が予測される生物種
Full Name YY1 transcription factor
Calculated molecular weight 414 aa, 45 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC037308
Gene symbol YY1
Gene ID (NCBI) 7528
RRIDAB_11182932
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.

Protocols

Product Specific Protocols
WB protocol for YY1 antibody 22156-1-APDownload protocol
IHC protocol for YY1 antibody 22156-1-APDownload protocol
IF protocol for YY1 antibody 22156-1-APDownload protocol
IP protocol for YY1 antibody 22156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Transl Med

Yin Yang 1 protein ameliorates diabetic nephropathy pathology through transcriptional repression of TGFβ1.

Authors - Pan Gao
humanWB

Mol Cell

Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.

Authors - Wenqi Xu
humanWB

Theranostics

KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.

Authors - Chengwan Zhang
  • KD Validated
humanChIP

Cancer Lett

MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.

Authors - Qi Sun
humanWB,IP,ChIP

Free Radic Biol Med

Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation.

Authors - Wentao Wang
mouseIHC

Cancers (Basel)

HPV8 Reverses the Transcriptional Output in Lrig1 Positive Cells to Drive Skin Tumorigenesis.

Authors - Adnan Shahzad Syed