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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YY1 Recombinant antibody (82712-3-RR)

    WB analysis using 82712-3-RR (same clone as 82712-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82712-3-RR (YY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82712-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Recombinant antibody (82712-3-RR)

    IF Staining of HepG2 using 82712-3-RR (same clone as 82712-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82712-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Recombinant antibody (82712-3-RR)

    IF Staining of HepG2 using 82712-3-RR (same clone as 82712-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82712-3-PBS in a different storage buffer formulation.

  • ChIP experiment of K-562 cells using YY1 Recombinant antibody (82712-3-RR)

    ChIP experiment of K-562 using 82712-3-RR (same clone as 82712-3-PBS)

    Chromatin was prepared from K-562 cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of YY1 Antibody (82712-3-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR This data was developed using the same antibody clone with 82712-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82712-3-RR

    Affinity and Kinetic Characterization of 82712-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82712-3-RR against Human YY1 were performed. The affinity constant is 0.835 nM.

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YY1 Recombinant antibody, PBS Only

YY1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ChIP, Indirect ELISA
Cat No. 82712-3-PBS
Clone No.1B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ChIP, Indirect ELISA

Synonyms

1B5, DELTA, Delta transcription factor, INO 80S, INO80S

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82712-3-PBS targets YY1 in WB, IF/ICC, ChIP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YY1 fusion protein Ag17792 相同性解析による交差性が予測される生物種
Full Name YY1 transcription factor
Calculated molecular weight 414 aa, 45 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC037308
Gene Symbol YY1
Gene ID (NCBI) 7528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25490
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.