Validation Data Gallery
Tested Applications
| Positive WB detected in | MDA-MB-231 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
10425-1-AP targets YY1AP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | YY1AP1 fusion protein Ag0692 相同性解析による交差性が予測される生物種 |
| Full Name | YY1 associated protein 1 |
| Calculated molecular weight | 75 kDa |
| Observed molecular weight | 75 kDa and 50 kDa |
| GenBank accession number | BC003500 |
| Gene Symbol | YY1AP1 |
| Gene ID (NCBI) | 55249 |
| RRID | AB_2218515 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H869 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
YY1AP1 (YY1 associated protein 1), also known as HCCA2 (hepatocellular carcinoma-associated protein 2) or YY1AP, is a 796 amino acid protein that localizes to both the nucleus and the cytoplasm and exists as seven alternatively spliced isoforms. We detected 75 kDa and 50 kDa two isoforms. YY1AP1 is expressed ubiquitously with notable expression in lung, brain, spleen, pancreas, small intestine, heart, colon and skeletal muscle, YY1AP1 interacts with and enhances the transcriptional activation activity of YY1. YY1AP1 is expressed at high levels in liver carcinomas, suggesting a role in tumor transformation and metastasis. YY1AP1 may play a role in cell cycle regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YY1AP1 antibody 10425-1-AP | Download protocol |
| IF protocol for YY1AP1 antibody 10425-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury. | ||
Eur J Pharmacol Cannabidiol represses miR-143 to promote cardiomyocyte proliferation and heart regeneration after myocardial infarction |