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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using YY2 Monoclonal antibody (66839-1-Ig)

    WB analysis of human kidney using 66839-1-Ig (same clone as 66839-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using YY2 Monoclonal antibody (66839-1-Ig)

    WB analysis of human placenta using 66839-1-Ig (same clone as 66839-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66839-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using YY2 Monoclonal antibody (66839-1-Ig)

    WB analysis of MCF-7 using 66839-1-Ig (same clone as 66839-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66839-1-Ig (YY2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66839-1-PBS in a different storage buffer formulation.

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YY2 Monoclonal antibody, PBS Only

YY2 Monoclonal Antibody for WB, ELISA
Cat No. 66839-1-PBS
Clone No.1C11A7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

Transcription factor YY2, Yin and yang 2, YY 2, YY2, YY2 transcription factor, Zinc finger protein 631, ZNF631

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66839-1-PBS targets YY2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen YY2 fusion protein Ag25373 相同性解析による交差性が予測される生物種
Full Name YY2 transcription factor
Calculated molecular weight 372 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC137217
Gene Symbol YY2
Gene ID (NCBI) 404281
RRIDAB_2882182
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15391
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

YY2 (Yin-Yang 2) encodes a zinc finger transcription factor similarity to the well-characterized YY1 that locating to nucleus. Both proteins recognize an identical DNA-binding motifs with a high homologous Zinc Finger region, and these transcription factors can act as activators or repressors when bound to DNA (PMID:26350623). It is reported that YY2 is placental mammal-specific and is absent in marsupial and non-mammalian vertebrate species (PMID:16377127). YYs are play an important role in multiple developmental processes , such as implantation, cell differentiation, X inactivation, imprinting and organogenesis (PMID:27191592). YY2 is expressed in kindey, liver, spleen and testicle but not in the colon (PMID:15087442). A 41kDa band could be detected.