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ZAK Polyclonal antibody
ZAK Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 14945-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue, HepG2 cells, human skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1500 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
Product Information
14945-1-AP targets ZAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZAK fusion protein Ag6747 相同性解析による交差性が予測される生物種 |
| Full Name | sterile alpha motif and leucine zipper containing kinase AZK |
| Calculated molecular weight | 91 kDa |
| Observed molecular weight | 52 kDa |
| GenBank accession number | BC001401 |
| Gene symbol | ZAK |
| Gene ID (NCBI) | 51776 |
| RRID | AB_10642698 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZAK antibody 14945-1-AP | Download protocol |
| IHC protocol for ZAK antibody 14945-1-AP | Download protocol |
| IF protocol for ZAK antibody 14945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Immunity Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.
| ||
Sci Signal Poly-GR repeats associated with ALS/FTD gene C9ORF72 impair translation elongation and induce a ribotoxic stress response in neurons | ||
bioRxiv Initiation of a ZAKα-dependent Ribotoxic Stress Response by the Innate Immunity Endoribonuclease RNase L | ||
Nat Commun Ribosome impairment regulates intestinal stem cell identity via ZAKɑ activation | ||
Cell Metab Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response | ||
Cell Death Dis Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Amy (Verified Customer) (08-15-2019) | Worked well with 5% milk in 0.2% PBST.Shows up well the beta isoform in mouse muscle, and alpha in heart.Did not work in Zebrafish despite the kinase domain being highly conserved between organisms.
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